Digitalized organoids for 3D structure and cellular-topology analysis
AI-based multilevel segmentation and topology analysis make organoid culture, imaging and screening more quantifiable.
Nature Methods
3DCellScope segments and analyzes topology at nuclear, cytoplasmic and whole-organoid scales to track structural change.
Imaging projects should predefine exposure, focal planes, segmentation quality, batch handling and manual-review rules rather than treating algorithm output as a biological conclusion.