PROTEOMICS WORKFLOWS

Proteomics

A traceable path from protein preparation and quantification to separation and functional interpretation.

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WORKFLOW MAP

From sample to evidence

01

Protein preparation

Control lysis, clarification, reduction, alkylation and digestion to preserve protein and peptide quality.

  1. Collect and keep cold
  2. Lyse and clarify
  3. Reduce, alkylate and digest
  4. Desalt and concentrate peptides

RisksDegradation, salt or detergent carryover, batch variation and low-input loss.

QCProtein amount, peptide recovery, blanks and batch records.

Recommended products

解脲脲原体（UU）核酸检测试剂盒（PCR荧光探针法） BFRT111M 96孔2.2mL深孔板 BFMH01A 沙眼衣原体（CT）核酸检测试剂盒（PCR荧光探针法） BFRT112M 6孔2.2ml深孔板 BFMH01B 淋球菌（NG）核酸检测试剂盒（PCR荧光探针法） BFRT113M 磁棒套（八联） BFMH02 乙型肝炎病毒（HBV）核酸定量检测试剂盒（PCR荧光探针法） BFRT114M 磁棒套（八联） BFMH02B 乙型肝炎病毒（HBV）核酸定量检测试剂盒（PCR荧光探针法） BFRT114D6 磁棒套（八联） BFMH02T 丙型肝炎病毒（HCV）核酸定量检测试剂盒（PCR荧光探针法） BFRT115M 96孔2.2mL深孔板 BFMH07 丙型肝炎病毒（HCV）核酸定量检测试剂盒（PCR荧光探针法） BFRT115D6 磁棒套（96联） BFMH08 人类ALDH2基因多态性核酸检测试剂盒（PCR荧光探针法） BFRT116M 磁棒套（96联） BFMH08E 人类CYP2C9基因多态性核酸检测试剂盒（PCR荧光探针法） BFRT117M 单样本单支底座固定套 BFMH10 10ul吸头 BFMH12 200ul吸头 BFMH13 1000ul吸头 BFMH14 100ul吸头 BFMH21 八联PCR管 BFMH15 八联PCR管 BFMH15B 24孔15ml深孔板 BFMH16 磁棒套（15ml） BFMH17 24孔10ml深孔板 BFMH18 磁棒套（10ml） BFMH19 研磨管 BFMH20-1 研磨管 BFMH20-2 研磨管 BFMH20-3 研磨管 BFMH20-4
Supporting consumablesResearch diagnostic reagents Configure this workflow

02

Quantification

Choose label-free, TMT or DIA strategies while controlling missingness, batch effects and quantitative bias.

  1. Design and randomize
  2. Plan standards or channels
  3. Acquire and calibrate
  4. Normalize and review missingness

RisksIon suppression, drift, ratio compression and insufficient power.

QCStandards, identifications, CV, missingness, FDR and batch drift.

Recommended products

96孔2.2mL深孔板 BFMH01A 6孔2.2ml深孔板 BFMH01B 磁棒套（八联） BFMH02 磁棒套（八联） BFMH02B 磁棒套（八联） BFMH02T 96孔2.2mL深孔板 BFMH07 磁棒套（96联） BFMH08 磁棒套（96联） BFMH08E 单样本单支底座固定套 BFMH10 10ul吸头 BFMH12 200ul吸头 BFMH13 1000ul吸头 BFMH14 100ul吸头 BFMH21 八联PCR管 BFMH15 八联PCR管 BFMH15B 24孔15ml深孔板 BFMH16 磁棒套（15ml） BFMH17 24孔10ml深孔板 BFMH18 磁棒套（10ml） BFMH19 研磨管 BFMH20-1 研磨管 BFMH20-2 研磨管 BFMH20-3 研磨管 BFMH20-4
Supporting consumables Configure this workflow

03

Separation

Use LC, ion mobility and fractionation to improve peak capacity and coverage in complex samples.

  1. Choose chromatography and gradient
  2. Fractionate or enrich
  3. Set DDA/DIA method
  4. Run system suitability

RisksRetention drift, co-elution, contamination and instrument-state changes.

QCRetention time, peak area, pressure, blanks and suitability samples.

Recommended products

96孔2.2mL深孔板 BFMH01A 6孔2.2ml深孔板 BFMH01B 磁棒套（八联） BFMH02 磁棒套（八联） BFMH02B 磁棒套（八联） BFMH02T 96孔2.2mL深孔板 BFMH07 磁棒套（96联） BFMH08 磁棒套（96联） BFMH08E 单样本单支底座固定套 BFMH10 10ul吸头 BFMH12 200ul吸头 BFMH13 1000ul吸头 BFMH14 100ul吸头 BFMH21 八联PCR管 BFMH15 八联PCR管 BFMH15B 24孔15ml深孔板 BFMH16 磁棒套（15ml） BFMH17 24孔10ml深孔板 BFMH18 磁棒套（10ml） BFMH19 研磨管 BFMH20-1 研磨管 BFMH20-2 研磨管 BFMH20-3 研磨管 BFMH20-4
Supporting consumables Configure this workflow

04

Functional analysis

Connect differential proteins, modifications, pathways and targets to cellular or disease phenotypes.

  1. Differential and enrichment analysis
  2. Pathway and network interpretation
  3. Functional validation
  4. Record reproducibility

RisksDatabase version, over-interpretation, confounding and missing orthogonal validation.

QCReference version, FDR, effect size, replicates and orthogonal assays.

Recommended products

解脲脲原体（UU）核酸检测试剂盒（PCR荧光探针法） BFRT111M 沙眼衣原体（CT）核酸检测试剂盒（PCR荧光探针法） BFRT112M 淋球菌（NG）核酸检测试剂盒（PCR荧光探针法） BFRT113M 乙型肝炎病毒（HBV）核酸定量检测试剂盒（PCR荧光探针法） BFRT114M 乙型肝炎病毒（HBV）核酸定量检测试剂盒（PCR荧光探针法） BFRT114D6 丙型肝炎病毒（HCV）核酸定量检测试剂盒（PCR荧光探针法） BFRT115M 丙型肝炎病毒（HCV）核酸定量检测试剂盒（PCR荧光探针法） BFRT115D6 人类ALDH2基因多态性核酸检测试剂盒（PCR荧光探针法） BFRT116M 人类CYP2C9基因多态性核酸检测试剂盒（PCR荧光探针法） BFRT117M
Research diagnostic reagents Configure this workflow

RESEARCH UPDATES

Recent research and workflow evidence