Compound screening · Workflow guide
High-throughput screening for 14-3-3 protein disruptors
BRET high-throughput screening combined with cellular efficacy and biophysical validation identifies modulators of 14-3-3 interactions.
Cell Death & Disease
The study establishes a BRET screen and carries hits into cell-death, selectivity and direct-binding assays, creating a screen-to-mechanism cascade.
Screening programs should monitor Z' factor, plate drift, false positives, repeat hits and dose-response behavior.
Read source articleDOI: 10.1038/s41419-025-08150-6